Immunohistochemical evaluation of l esions in the gastrointestinal tract of b uffalo ( Bubalus bubalis ) calves orally exposed to Pasteurella multoci da B:2

Abstract

The g astrointestinal lesions and bacterial distribution of buffalo calves were evaluated histologically using immunoperoxidase, following oral exposure to wild - type Pasteurella multocida B:2 at 10 9 cfu/mL in ph osphate buffered saline. The lesions were basically of mild to severe mucohaemorrhagic abomasitis and enteritis.The lesions were confirmed to be associated with the inoculated P. multocida B:2, using the immunoperoxidase technique. P. multocida B:2 antige n was detected not only in the bacterial clusters in the gastric pits, intestinal epithelia and capillaries, Brünner’s glands and Crypt of Lieberkühn but was also seen interacting with infiltrating neutrophils and macrophages intracellularly and on the sur face of erythrocyte in congested vessels and haemorrhages. We observed higher localization and distribution of the immunoperoxidase reaction with increased severity of lesions along the gastrointestinal tract . T his suggest intensity increases with increase d amount of P. multocida B:2 or antigen in the tissue, which possibly lead s to increase tissue damage.