Abstract:
The g
astrointestinal lesions and bacterial distribution
of buffalo calves
were evaluated histologically using
immunoperoxidase, following oral exposure to wild
-
type
Pasteurella multocida
B:2 at 10
9
cfu/mL in ph
osphate
buffered saline. The lesions were basically of mild to severe mucohaemorrhagic abomasitis and enteritis.The
lesions were confirmed to be associated with the inoculated
P. multocida
B:2, using the immunoperoxidase
technique.
P. multocida
B:2 antige
n was detected not only in the bacterial
clusters in the gastric pits, intestinal
epithelia and capillaries, Brünner’s glands and Crypt of Lieberkühn but
was
also seen interacting with
infiltrating neutrophils
and
macrophages intracellularly and on the sur
face of erythrocyte in congested vessels
and haemorrhages. We observed higher localization and distribution of the immunoperoxidase reaction with
increased severity of lesions along the gastrointestinal tract
. T
his suggest intensity increases with increase
d
amount of
P. multocida
B:2 or antigen in the tissue, which possibly lead
s
to increase tissue damage.
