Abstract:
Euphorbia balsamifera
plant belongs
to the family
of
Euphorbiaceae
.
Leaves,
stems and root
s
of
Euphorbia bal
samifera
were
extracted
using ethanol and the
extracts
were
screened
for phytochemical components.
The phytochemical results
indicated that the plant
con
tained
tannins,
flavonoids,
saponins,
steroid
s
,
glycosides,
terpenoid
s
,
alkaloid
s
,
and anthraquinones
.
E
thanol extract of leaves
and its fractions were
tested for an
tibacterial activity
using agar well diffusion
method
and were
found
to show inhibitory activity against
Escherich
i
a col
i
,
Staphylococcus
aureus,
Micrococcus species
and
Pseudomonas aeruginosa
.
The
sensitivity test results showed
highest activity of 25
mm zone of inhibition at
120mg/ml
of
chloroform
fraction
against
M
icrococcus species
.
The MIC and MBC
of the chloroform fraction were 5
mg/ml
and 7
mg/ml
respectively
.
T
he
TLC of
chloroform fraction
of leaves
sho
wed six
components of different R
f
value
using
benzene:
ethyl
acetate (3:1) solvent system.
The
chloroform fract
ion was further
subjected to column chromatography
,
after which preparative TLC was carried
out
on
the fraction obtained from column
.
The
isolated compound
was
subjected to
IR,
UV and GC
-
MS S
pectroscopy which reve
aled
the presence of
(
6H
-
Indolo[3,2,1
-
de
][1,5]naphthyridin
-
6
-
one)
and fatty acid
in the isolated compound.
Therefore the
bioactive
of
Euphorbia balsamifera
leaves
can be
served as
a lead for the
development of new pharmaceutical therapeutic needs
