INHIBITION OF CAMEL LENS s-CRYSTALLIN/NADPH:QUINONE OXIDOREDUCTASE ACTIVITY BY 2,~, 6-TRINITROBENZENE SULFONIC ACID (TNBS)

Abstract

Camel lens s-crystallin/NADPH:quinone oxidoreductase activity was inhibited by trinitrobenzene sulfonic acid (TNBS) with NADPH as an electron donor and dichlorophenolindophenol (DCIP) as an electron acceptor in a time-independent but concentration dependent manner. The rco of TNBS was 30 ulvl. The inhibition was noncompetitive with respect to DCIP as deduced by Lineweavcr-Burk plots. The estimated inhibition constant (Ki) was 35 uM. The inhibition of s-crystallin by TNBS will give an insight into understanding the catalytic activity of this enzyme whose function. nature of active site and importance are yet to be fully established.