Abstract:
This experiment was aimed at investigating the level of tissue regeneration f
ollowing sanguineous reperfusion post ligation
of the spermatic cord in dogs. A total of 5 adult Nigerian local dogs conditioned for 3 weeks, laboratorily evaluated twice
weekly prior to surgery were maintained for 1month. Dogs were aseptically operated un
der general anaesthesia. A
prescrotal mid
-
ventral incision was made to access the tunics and ligated (with 2
-
0 chromic catgut) as in closed castration
in canines. Two ligatures were placed proximal and distal on the tunics of the spermatic cord 1.5cm apa
rt to each other to
completely occlude the vascular supplies to the testicles. Skin closures were by a single horizontal suture stitch.
A second operation was performed to undo the ligatures placed on the spermatic cords as follows: Dog 1(24hours), Dog 2
(72hours), Dog 3(96hours), and Dog 4 (120hours) and Dog 5 (144hours) to allow for reperfusion. A third operation was
performed two weeks later to harvest the testicles together with their spermatic cords but noticed that all ligated testicles
were complet
e necrotized and degenerated. Tissue necrosis was suggestive of severe ischaemia due to the fact that the
scrotal sac and its contents are highly sensitive and respond to pain extremely fast. From our finding, it is realized that t
he
resistibility of these
organ to injury is very low and dies very fast when its vascular supply is interrupted.