Microsecond Laser Flash Photolysis of the CO-Complex of Bovine Heart Cytochrome c Oxidase

Abstract

the fully reduced and mixed valence enzyme was prepared as described by Bicker et al. (1984). Kinetics of CO rebinding subsequent to Laser photolysis was studied at 430nm by using a 20ns pulsed at 532nm vertically polarized Laser beam of 2mJ energy. The results suggest differences in photodynamic behaviour of the fully reduced and mixed valence forms of the enzyme. The most important implication of this, is at the conformational changes associated with the reduction of the low spin cytochrome a affects the ligation dynamic of the oxygen reduction site via heterotropic interaction.