KINETIC STUDIES OF RHODANESE IN SOME TISSUES OF SOKOTO RED GOAT

Saidu, Y; Alawaye, G.A; Bilbis, L.S; Abbas, A.Y

dc.contributor.author	Saidu, Y
dc.contributor.author	Alawaye, G.A
dc.contributor.author	Bilbis, L.S
dc.contributor.author	Abbas, A.Y
dc.date.accessioned	2017-10-31T09:13:51Z
dc.date.available	2017-10-31T09:13:51Z
dc.date.issued	2005
dc.identifier.uri	http://hdl.handle.net/123456789/253
dc.description.abstract	Cyanide:thiosulphate sulphur transferase (rhodanese) activity was assayed spectrophotometrically in some tissues of Sokoto red goat. The liver enzyme was subjected to further kinetic studies. Other tissues studied were kidney, heart and lungs. The order of the activity of the enzyme in these tissues was liver (4.13!lmol SCNmin- !g-! fresh tissue) > Kidney (2.3 umol SCNmin'!g'! fresh tissue) »heart (1.4 umol SCNmin-!g'! fresh tissue) > lungs (1.10 umol SCNmin'!g'! fresh tissue). Kinetic studies of the liver enzyme indicated an optimum pH and temperature of 8.0 and 40°C respectively. The activation energy was 17.9 Kl/mol. Preincubation of the enzyme extract in cyanide inhibited its activity while preincubation in thiosulphate enhances its activity. These results provide an insight regarding physiological role and mechanism of action of rhodanese in the tissues of Sokoto red goat.	en_US
dc.language.iso	en	en_US
dc.publisher	Bulletin of SAN	en_US
dc.subject	Department of Biochemistry	en_US
dc.title	KINETIC STUDIES OF RHODANESE IN SOME TISSUES OF SOKOTO RED GOAT	en_US
dc.type	Article	en_US