Abstract:
Fresh leaves of Cassia alata Linn were extracted using different solvents first with water,
then methanol then n-hexane and lastly with acetone. On comparison between methanol extract and
extracts of other solvents it was revealed that methanol gave the maximum extraction, this was
evident during the chromatographic separation as it gave large number of fractions. Chromatographic
method was used to separate the different components of the extract. The crude extract as well as the
chromatographic fractions were tested for antibacterial activity using disc diffusion method. Among
the fractions, only one component show antibacterial activity on Staphylococcus aureus. The active
chromatographic fraction revealed the presence of steroids
